Introduction

The market for therapeutic antibodies has dramatically expanded over the past decades since their first approval in 1986.

Antibodies, which are proteins, form their structure and exert their activity through a complex series of non-covalent bonds and may lose their activity due to various external stimuli.

Therefore, the evaluation of structural stability is extremely important in the development and formulation of candidate antibodies.

Thermodynamic stability of antibodies is generally evaluated by DSC (differential scanning calorimetry) and circular dichroism (CD) spectroscopy.

Circular Dichroism spectroscopy is an easy and rapid method for obtaining information on the secondary and tertiary structure of proteins in solution and can be used to directly evaluate the protein structural change caused by heat.

Recently, Micsonai et al. developed the BeStSel algorithm that can accurately estimate the secondary structure composition from the CD spectrum by taking into account the parallel-antiparallel orientation of the β-strands and the twist of the antiparallel β-sheets.

BeStSel has the following features:

High estimation accuracy for a wide range of proteins, including β-structure-rich-proteins such as antibodies

Providing eight types of secondary structure information

A capability to predict the protein fold following the CATH classification

An open web server

While many academic researchers use the BeStSel web server, researchers in biopharma who need to work in a GxP environment have not been able to benefit from BeStSel.

To make BeStSel accessible to biopharma, JASCO developed Spectra Manager™ Ver.2.5 CFR BeStSel as an add-in software for Spectra Manager™, a control and analysis platform for CD spectrometers, which is compatible with GxP.

Epson M100 Resetter Nosware _top_ Official

printer has suddenly stopped working and is flashing service lights, you've likely encountered the "Waste Ink Pad" or "Service Required" error. This is a built-in safety feature that halts printing when the internal ink pads are calculated to be full. Using a software tool like the Epson M100 Resetter

"The printer's ink pads are at the end of their service life. Please contact Epson Support." epson m100 resetter nosware

Have you successfully used a nosware resetter on your Epson M100? Share your experience in the comments below (if on a blog) or in printer forums. Your insight could help others avoid expensive repairs. printer has suddenly stopped working and is flashing

An Epson resetter is a small software utility (or sometimes a manual tool) that connects to your printer via USB. Its only job is to reset the . Please contact Epson Support