The manual’s back cover lists a QR code for booking training modules. Typical courses:
15°, 90°, and 175° (Backscattering) for optimized sizing of different sample types. anton paar litesizer 500 manual
| Feature | Specification | | :--- | :--- | | | PALS (Phase Analysis Light Scattering) for Zeta; DLS for Size | | Particle Size Range | 0.3 nm – 10 µm | | Zeta Potential Range | -600 mV to +600 mV | | Molecular Weight | 300 Da to 20 MDa | | Laser Wavelength | 658 nm (40 mW) | | Temperature Range | 0 °C to 120 °C | The manual’s back cover lists a QR code
If your Litesizer 500 is equipped with the optional Part 11 module, the manual’s supplement explains: The stands as a gold standard instrument for
In the world of particle characterization and colloidal science, precision is not a luxury—it is a necessity. The stands as a gold standard instrument for analyzing nanoparticles, emulsions, suspensions, and proteins. It measures three critical parameters: particle size (via Dynamic Light Scattering, DLS), zeta potential (via Electrophoretic Light Scattering, ELS), and molecular mass.
| | Answer (Manual Reference) | |---|---| | Q: Can I measure organic solvents like hexane? | Yes, but you must manually enter viscosity and refractive index (RI). The manual provides a table for common solvents (page 142). For hexane: viscosity = 0.3 cP, RI = 1.375. | | Q: My zeta potential variability is high (>20%). Why? | Likely electrode contamination or bubble formation. Re-clean per Chapter 6.8. Also, ensure sample conductivity > 0.5 mS/cm (add 0.1 mM KCl if needed). | | Q: How often must I recalibrate? | Annually, by an Anton Paar service engineer. However, the manual suggests a weekly check using a 100 nm standard; if the Z-Average drifts >5% or PdI >0.1, perform a "Calibration Reset" via the Service Menu (password-protected). | | Q: Can I measure particles >10 µm? | No, DLS accuracy drops sharply. The manual states that results >10 µm should be reported as “>10 µm (limitations of technique)” – use laser diffraction instead. | | Q: What does "Outlier Removal" do? | Found in Analysis Settings. It removes runs where count rate deviates by >30% from median. Useful for aggregating samples. Manual warns: do not use for monodisperse polymers. |